Molecules more compact as opposed to pore size penetrate the beads and choose for a longer time to elute, though larger molecules are excluded with the pores and elute much more immediately. It is commonly used to determine the molecular pounds distribution of polymers and to individual biomolecules like proteins.For reliability and best observe, U… Read More


This method is based around the principle that different molecules absorb UV light-weight at various wavelengths and intensities.Mass spectra plots present the relative abundance of ions and so are utilised to ascertain molecular framework and composition.UV spectroscopy is a method employed to analyze the composition of the sample by measuring its… Read More


Quite a few labor intensive and time-consuming techniques are available for RNA isolation, purification and quantification. Quantification of RNA samples is done by measuring their absorption at 260 nm, when the quality and integrity of RNA samples are typically based on gel electrophoresis accompanied by ethidium bromide visualization (1–3).What… Read More


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